dissolving cyst form with RX

Discussion in 'Lyme Disease Archives' started by victoria, Mar 24, 2010.

  1. victoria

    victoria New Member

    This is interesting, about using the brand name Plaquenil... My son was rx'd Mepron for babesia... double duty perhaps. This abstract was included on one of the lists I'm on, may be helpful...

    An in vitro study of the susceptibility of mobile and cystic forms of Borrelia burgdorferi to hydroxychloroquine.
    Brorson O, Brorson SH.

    Department of Microbiology, Vestfold Sentralsykehus, Tønsberg, Norway.

    In this work the susceptibility of mobile and cystic forms of Borrelia burgdorferi to hydroxychloroquine (HCQ) was studied.

    The minimal bactericidal concentration (MBC) of HCQ against the mobile spirochetes was > 32 microg/ml at 37 degrees C, and > 128 microg/ml at 30 degrees C.

    Incubation with HCQ significantly reduced the conversion of mobile spirochetes to cystic forms. When incubated at 37 degrees C, the MBC for young biologically active cysts (1-day old) was > 8 microg/ml, but it was > 32 microg/ml for old cysts (1-week old).

    Acridine orange staining, dark-field microscopy and transmission electron microscopy revealed that the contents of the cysts were partly degraded when the concentration of HCQ was > or = MBC.

    At high concentrations of HCQ (256 microg/ml) about 95% of the cysts were ruptured. When the concentration of HCQ was > or = MBC, core structures did not develop inside the cysts, and the amount of RNA in these cysts decreased significantly.

    Spirochetal structures inside the cysts dissolved in the presence of high concentrations of HCQ. When the concentration of HCQ was > or = MBC, the core structures inside the cysts were eliminated.

    These observations may be valuable in the treatment of resistant infections caused by B. burgdorferi, and suggest that a combination of HCQ and a macrolide antibiotic could eradicate both cystic and mobile forms of B. burgdorferi.

    PMID: 12102233 [PubMed - indexed for MEDLINE]

    The herbs that appear to help dissolve biofilm include nattokinase, serrapeptase and grapefruit seed extract, tho I'm not sure about studies regarding this.
    [This Message was Edited on 03/24/2010]
  2. ChuckNBerkeley

    ChuckNBerkeley New Member

    Here is a link to the whole article as a pdf. Includes pictures.

    Brorson also did; metronidazole and tinidazole for cyst forms and tigecycline for "round-bodies".

    As for Grapefruit Seed Extract (GSE). Brorson pdf;

    The highest GSE concentrations made the bacteria and cysts disappear completely, leaving only small uncharacteristic fragments; at lower GSE-levels the membranes showed herniation and disruption, and the contents had leaked out. The MBC was strongly dependent on the length of the incubation. GSE was very active even for very short incubation times, in agreement with previous results [7]. The MBC obtained by DFM for the motile bacteria agreed well with the TEM results. Presence of GSE reduced the conversion from spirochetes to cysts when the susceptibility testing was performed in distilled water. This study was performed in vitro and further studies are needed to demonstrate eventual effects in vivo. From our results it will be rational to test the hypothesis that a combination of GSE and antibiotics will be efficient in the treatment of resistant Lyme borreliosis.
    Ø. Brorson, S.-H. Brorson
    End quote
    [This Message was Edited on 03/24/2010]
  3. victoria

    victoria New Member

    Thanks Chuck!