Heat shock protein response to exercise in ME/CFS

Discussion in 'Fibromyalgia Main Forum' started by tansy, Nov 27, 2008.

  1. tansy

    tansy New Member

    Differential heat shock protein responses to strenuous standardized
    exercise in chronic fatigue syndrome patients and matched healthy controls.

    Journal: Clin Invest Med. 2008 Dec 1;31(6):E319.

    Authors: Thambirajah AA, Sleigh K, Stiver HG, Chow AW.

    NLM Citation: PMID: 19032901

    Purpose: Since physical exertion is known to exacerbate the symptoms
    of chronic fatigue syndrome (CFS) and metabolic changes and including
    oxidative stress can modulate heat shock protein (HSP) expression
    responses, we sought to determine whether HSP expression is altered
    in CFS patients before and after exercise.

    Methods: HSP27, HSP60, HSP70 and HSP90 expression from 6 CFS patients
    and 7 age- and sex-matched controls were examined by western blot
    analysis of peripheral blood mononuclear cells immediately before,
    after, and at 1 day and 7 days following a standardized treadmill exercise.

    Results: Basal HSP27 was higher among CFS patients than in controls
    (0.54 +/- 0.13 vs. 0.19 +/- 0.06, mean +/- SEM; P < 0.01). In
    addition, these levels in CFS patients decreased immediately
    post-exercise (0.25 +/- 0.09; P < 0.05) and remained below basal
    levels at day 1 post-exercises (0.18 +/- 0.05; P < 0.05). P < 0.05).
    This declining expression of HSP27 during the post-exercise period
    among CFS patients was confirmed by one-way ANOVA analysis with
    repeated measures (P < 0.05). In contrast, HSP27 levels remained
    relatively constant following exercise among control subjects.
    Similar patterns of declining HSP levels in CFS patients were also
    observed for HSP60 (0.94 +/- 0.40 vs. 1.32 +/- 0.46; P < 0.05), and
    for HSP90 (0.34 +/- 0.09 vs. 0.49 +/- 0.10; P < 0.05) at day 7
    post-exercise compared with basal levels, respectively. In contrast,
    HSP60 levels in control subjects increased at day 1 (1.09 +/- 0.27)
    and day 7 (1.24 +/- 0.50) post-exercise compared to corresponding
    levels immediately post-exercise (0.55 +/- 0.06) (P < 0.05, respectively).

    Conclusion: These preliminary findings suggest an abnormal or
    defective adaptive response to oxidative stress in CFS, and raise the
    possibility that HSP profiling may provide a more objective biologic
    marker for this illness.