help with labs please

Discussion in 'Lyme Disease Archives' started by 3gs, Jan 19, 2009.

  1. 3gs

    3gs New Member

    Hi i posted this on the Fibro board .

    I had an equivocal test for Lyme several months ago. I asked doc to run a Western Blot. Doc says negative but there are some bands that show postive. Could you please help me understand these as my doc is not good at this.

    IgG P41 Ab PRESENT

    I asked doc to run other tests but he only ran cbc and I have to go back for coristol am. He did give me Doxycin(antibotic at my request but havent taken as he is not very supportive or knowledgable). I do also have Fibro ME post polio etc.

    Thanks for all your help
    [This Message was Edited on 01/19/2009]
  2. munch1958

    munch1958 Member

    The following two reports by Allen Steere and Yale state that if a person has band 41 (or flagellin), Lyme symptoms, and they do not have severe periodontal disease or syphilis, they have Lyme borreliosis:

    http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=423723&blobtype=pdf

    1) Allen Steere in 1986, when he developed the first CDC Method to diagnose Lyme, recommended: ” Perform serial Western Blots to look for changing and expanding IgM and IgG antibodies,” since Lyme is a borrelisis, a relapsing fever, and the changing antibodies is a reflection of the varying antigens- and that, THIS CHANGING phenomenon means β€œthe spirochete remains alive throughout the illness.”

    In that full text report, Steere said one can distinguish between Lyme and syphilis, when one only sees band 41 (anti-flagellar antibody) in a person complaining of Chronic Fatigue Syndrome or Fibromyalgia.

    2) Yale and CT Agricultural experiment Station- the full pdf:

    http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8788993

    Use of recombinant antigens of Borrelia burgdorferi in serologic tests for diagnosis of lyme borreliosis.

    Magnarelli LA, Fikrig E, Padula SJ, Anderson JF, Flavell RA.

    Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504, USA.

    Recombinant antigens of outer surface proteins (Osps) OspA, OspB, OspC, OspE, and OspF of Borrelia burgdorferi sensu stricto and of p41-G, an antigenic region of flagellin of this spirochete, were tested with human sera in class-specific and polyvalent enzyme-linked immunosorbent assays (ELISAs). In analyses for immunoglobulin M (IgM) antibodies, 18 (85.7%) of 21 serum samples from persons who had been diagnosed as having Lyme borreliosis on the basis of the presence of erythema migrans reacted positively in ELISAs with one or more Osp antigens or the p41-G antigen. Eleven serum samples contained antibodies to OspC antigen, and of these, six also reacted to the p41-G antigen and to one or more of the other recombinant antigens. The remaining five serum samples reacted solely to OspC (n = 4) or to OspC plus OspA and OspE without reactivity to p41-G (n = 1). In analyses for IgG antibodies, seropositivity was comparable to that of IgM analyses and was marked by predominant reactivity to p41-G, OspC, and OspF. Similarly, all 21 serum samples were positive in polyvalent and class-specific ELISAs with whole-cell B. burgdorferi. Minor cross-reactivity was noted when sera from persons who had syphilis, periodontitis or other oral infections, or rheumatoid arthritis were tested with OspC, OspE, OspF, and p41-G. With relatively high degrees of specificity, ELISAs with recombinant antigens, particularly OspC and p41-G, can help to confirm B. burgdorferi infections.
    PMID: 8788993 [PubMed - indexed for MEDLINE]

    See Dr Kent Holtorf's page on Lyme -- Why this info didn't trickle down to all FFC docs is what I want to know:

    http://www.hormoneandlongevitycenter.com/lymedisease/