Lyme ARTHRITIS Research article

Discussion in 'Lyme Disease Archives' started by victoria, Jan 7, 2008.

  1. victoria

    victoria New Member

    Borrelia burgdorferi Basic Membrane Proteins A and B Participate in the Genesis of Lyme Arthritis


    ABSTRACT

    Lyme arthritis results from colonization of joints by Borrelia burgdorferi and the ensuing host response. Using gene array based differential analysis of B. burgdorferi gene expression and quantitative reverse trancription-polymerase chain reaction, we identified two paralogous spirochete genes, bmpA and bmpB, that are preferentially up-regulated in mouse joints compared with other organs.

    Transfer of affinity-purified antibodies against either BmpA or BmpB into B. burgdorferi infected mice selectively reduced spirochete numbers and inflammation in the joints. B. burgdorferi lacking bmpA/B were therefore generated to further explore the role of these proteins in the pathogenesis of Lyme disease.

    B. burgdorferi lacking bmpA/B were infectious in mice, but unable to persist in the joints, and they failed to induce severe arthritis.

    Complementation of the mutant spirochetes with a wild-type copy of the bmpA and bmpB genes partially restored the original phenotype.

    These data delineate a role for differentially produced B. burgdorferi antigens in spirochete colonization of mouse joints, and suggest new strategies for the treatment of Lyme arthritis.

    Published online 31 December 2007
    doi:10.1084/jem.20070962 The Journal of Experimental Medicine

    http://www.jem.org/cgi/content/abstract/jem.20070962v1

    Utpal Pal(1), Penghua Wang(1), Fukai Bao(1), Xiuli Yang(2), Swapna Samanta(1), Robert Schoen(1), Gary P. Wormser(3), Ira Schwartz(4), and Erol Fikrig(1)

    (1) Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520
    (2) Department of Veterinary Medicine, University of Maryland, College Park, MD 20742
    (3) Division of Infectious Diseases, Department of Medicine, and
    (4) Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595

  2. highcotton

    highcotton New Member

    I wonder how closely the mouse antibodies are to human antibodies? The results of the study are really amazing.

    now, if only we can get funding for more research like this...yeah, right.


    thanks for posting this!