My Ingenex Test Results: Need Interpretation

Discussion in 'Lyme Disease Archives' started by Elisa, Nov 17, 2007.

  1. Elisa

    Elisa Member

    I am posting this from the CFS/FM board. As background I have had a fever for about 5 months (99.5-100.7 with spikes up to 101)...

    My Dan doctor allowed me to get these tests from Igenex.

    Luyme Multiplex PCR
    Multiplex B Burgdorferi - Whole Blood
    Genomic - Negative
    Plasmid - Negative


    Lyme IgG Western Blot

    18 kDA --
    22 kDA --
    **23-25 kDa --
    28 kDa --
    30 kDa --
    **31 kDa --
    **34 kDa IND
    **39 kDa --
    **41 kDa IND
    45 kDa --
    58 kDa +
    66 kDa -
    73 kDa -
    **83-93 kDa -


    Lyme IgM Western Blot

    18 kDA --
    22 kDA --
    **23-25 kDa --
    28 kDa --
    30 kDa +
    **31 kDa IND
    **34 kDa --
    **39 kDa --
    **41 kDa IND
    45 kDa --
    58 kDa +
    66 kDa -
    73 kDa -
    **83-93 kDa -



    IFA, B Burgdorferi G/M/A <1:40 (me)
    Key:
    <1:40 = negative
    1:40 IND
    = or >1:80 positive


    Luyme Multiplex PCR
    Multiplex B Burgdorferi - Serum
    Genomic - Negative
    Plasmid - Negative

    That's all the tests I had...

    Hi Cindy - I have not been diagnosed officially with Lyme. My Dan doctor isn't a lyme literate - so he said all is negative.

    Hi Munch - love to hear your expert opinion!

    Anyone with Lyme experience feel free to comment - I know nothing - but want to learn...

    Elisabeth


    [This Message was Edited on 11/17/2007]
  2. cherylsue

    cherylsue Member

    Your lyme tests do appear negative, even by Igenex standards. However, Lyme is a clinical diagnosis. Your body may not have developed antibodies yet, but you may still have the disease. Find a LLMD.

    That's my opinion.

    CherylSue
  3. munch1958

    munch1958 Member

    Because of the INDs at some Lyme specific bands which are the double starred bands I'd say it's possible. I'd seek out a LLMD.

    From my own experience with the strain of Borrelia that I have band 30 has lots of neuro symptoms. Dr C would like these two bands folded in together like **30-31 kDa.

    I also have band 58 positive. The FFC told me it was nothing but it's probably a borrelia heat shock protein.

    IGG:

    **34 kDa IND
    **41 kDa IND
    58 kDa +

    IgM:

    30 kDa +
    **31 kDa IND
    **41 kDa IND
    58 kDa +

    Here's a link to Lyme bands around the world or what other countries consider significant bands.

    And The Bands Played On - Western blot serological test for Lyme disease

    http://www.geocities.com/HotSprings/Oasis/6455/western-blot.txt

    I like the title reference to the movie about Aids "And the Band Played On." I just read that Lyme infections are 5 times more common than AIDS.

    Source:
    http://www.hormoneandlongevitycenter.com/lymedisease/

    REVISED CRITERIA WITH WB

    1. IGG WB: 2 specific band criteria has demonstrated improved sensitivity and maintained specificity

    2. Can diagnosis Lyme if any one band (IgG or IgM) of 18, 23, 28, 39 or 58 kDa or if any 2 or more of the following bands are present; 30, 45,41 and 93

    3. If negative or require further confirmation, can obtain IGENEX WB (adds specific bands of 31, 34 an 83, which are typically seen in chronic disease)

    4. Positive if any one band of 18, 23, 28,31,34, 39, 58 or 83

    5. If positive for Borrelia on any test, consider testing for neurotoxins

    6. Consider testing for co-infections (discussed below)

    7. Check for coagulation defect (See Hypercoaguable State in CFS and FM)


  4. bunnyfluff

    bunnyfluff Member

    Igenex vs Quest explained 09/25/07 06:58 AM

    I know that many of you have tested negative on the standard Quest Lyme tests, and don't see the point in spending the extra $$ on Igenex, or understand how the 2 tests vary. I have recently had tthem done, and my LLMD was kind enuf to explain in depth the tests, as well as the results. Here is what I have learned:

    Lyme IgG Western Blot shows and old, or chronic infection. IgM shows new infection.

    Quest testing leaves out the 2 most specific bands: #31 & #34. These bands are SO SPECIFIC to Lyme, the first human Lyme vaccine was made from #31, and the 2nd from #34!! These very important bands are NOT REPORTED in standard commercial Lyme tests!

    Laboratories that use FDA approved kits are RESRICTED from reporting all of these bands, as they must abide by the rules of he manufacturer. These rules are set up in accordance wih the CDC's surveillance criteria, and inrease the risk of false negative resuls.

    The little ++ indicates the amount of reaction. Each + counts for 10% probability that you have Lyme. So, when you count your + signs, you can have 9, or 90%, and the CDC will give a neg. result because it has to be 100% for that to pass their "standard" for "surveillance".

    Here are the bands explained:



    If the patient is highly symptomatic of Lyme, there is actually no point in doing the ELISA or EIA serum tests, as they do not have the sensitivity or specificity of the Western Blot that is needed to have a prayer of detecting Borrelia burgdorferi (Bb), the organism that causes Lyme disease.


    Contrary to what many insurance companies believe, the IgG and IgM Western Blot for Lyme disease are not the same test. Some companies will deny one and pay the other, claiming they are the same test or duplicative of one another. IgG and IgM are two completely different antibodies.

    IgM antibodies are the first antibodies to be produced in the body in response to an infection, and is produced in great quantity. IgM antibodies are large, up to six times larger than the IgG antibodies. IgM antibodies, when present in high numbers, represent a new active infection or an existing infection that has become reactivated. Over time, the number of IgM antibodies will decline as the active infection is resolved.

    IgG antibodies are produced once an infection has been going on for a while, and may be present after the infection has been resolved. Generally speaking, the presence of IgG antibodies to an organism when accompanied by a negative IgM test for the same organism means that the person was exposed to that organism at one time and developed antibodies to it, but does not have a current active infection of that organism. When it comes to Borrelia burgdorferi (Bb), the organism responsible for Lyme disease, that is not necessarily the case.

    To recap, depending on the numbers,

    IgM is a sign of a current infection.
    IgG is a sign of a current infection, or of a past exposure to or past infection by the organism.
    Bb can hide in the brain and cerebral spinal fluid (CSF) and by altering its surface proteins, can remain invisible to the immune system for a long period of time. Once the immune system figures out what it is and starts making antibodies to it, it shifts is surface proteins once again, fooling the body into thinking the infection is over.

    Bb can also turn itself into undetectable cysts and various other forms (called L-forms) which also help it elude the immune system. If the immune system can't see it, the immune system can't make and, or only insufficient antibodies, which all contribute towards making the organism impossible to detect by any testing methodology, including WB. Thus, blood and urine tests for Bb can be negative, even if the patient is "challenged" by being given high dose injections of antibiotics to try to trigger a reaction from or partial die-off of Bb that will cause it to show up in the blood or urine.






    Band IgG
    IgM
    Band Definition

    18 kDa.#
    . p18 flagellin fragment
    22 kDa . . Immunogenic integral membrane lipoproteins. Cross-reactive with other spirochetes/bacteria. Depending on source, may be specific for Bb or cross-reactive. [Coleman]
    23-25 kDa @ # @ # OspC. 25 kDa is specific for Bb
    28 kDa .# . OspD, Oms28. Specific for Bb
    30 kDa # . OspA substrate binding protein
    31 kDa @ @ OspA
    34 kDa @ @ OspB. Specific for Bb
    37 kDa .. .. p37, FlaA gene product. Specific for Bb
    39 kDa @ # @ # BmpA. Specific for Bb
    41 kDa
    @ # @ # FlaB
    45 kDa .# . [Flisiak]; appears for HGE [Ravyn]
    58 kDa # . .
    66 kDa # . p66 Oms66 Hsp outer/integral membrane protein
    73 kDa ... ... .
    83 kDa .. .. p83 high molecular mass protein. Specific for Bb
    93 kDa @ # # an immunodominant protoplasmic cylinder antigen, associated with the flagellum. Specific for Bb

    Abbreviations:
    Bb Borrelia burgdorferi
    Bmp Bacterial membrane protein
    Fla Flagellin
    HGE Human granulocytic ehrlichiosis
    kDa kilodalton = molecular weight
    Oms Outer membrane-spanning
    Osp Outer surface proteins
    p Protein


    I hope this helps some of you understand your past testing.

    There are 5 subspecies of Lyme (Bb), over 100 STRAINS in the US, and over 300 WORLDWIDE! So, at least, try to get the best testing done that is available to rule this out.

    I am now in recovery, where in March I was near disability!! This will not be a simple process, but a least one with hope and healing.

    Bunny