New more sensitive blood test for lyme borreliosis

Discussion in 'Fibromyalgia Main Forum' started by tansy, Sep 4, 2005.

  1. tansy

    tansy New Member

    J Clin Microbiol. 2005 Aug;43(8):3602-9.

    Improvement of Lyme borreliosis serodiagnosis by a newly developed
    recombinant immunoglobulin G (IgG) and IgM line immunoblot assay and
    addition of VlsE and DbpA homologues.

    Goettner G, Schulte-Spechtel U, Hillermann R, Liegl G, Wilske B,
    Fingerle V.

    Max von Pettenkofer-Institut fur Hygiene und Medizinische
    Mikrobiologie, Ludwig-Maximilians-Universitat Munchen,
    Pettenkoferstr. 9a, D-80336 Munich, Germany.

    We developed and evaluated a recombinant Borrelia line immunoblot
    assay based on 18 homologues of seven different antigens, i.e.,
    p100, p58, p41i, BmpA, VlsE, OspC, and DbpA. Each recombinant
    antigen can be detected separately and is distinct even from
    homologues with identical molecular weights. This blot was compared
    to the recently described recombinant Borrelia Western immunoblot
    assay (U. Schulte-Spechtel, G. Lehnert, G. Liegl, V. Fingerle, C.
    Heimerl, B. J. Johnson, and B. Wilske, J. Clin. Microbiol. 41:1299-
    1303, 2003).

    To verify sensitivity and specificity, both blots were
    evaluated for reactivity with Borrelia-specific immunoglobulin G
    (IgG) and IgM antibodies with 85 sera from patients with different
    manifestations of Lyme borreliosis and 110 controls. According to
    European interpretation criteria for Borrelia Western blots, which
    define a serum as positive when it recognizes at least two bands,
    sensitivity increased significantly from 70.6% (Western blot) to
    84.7% (line blot) for IgG (P = 0.042) and from 40.0% (Western blot)
    to 73.8% (line blot) for IgM (P < 0.005).

    The increased sensitivity for IgG detection is due to the new line blot technique, whereas the improvement in detection of IgM is mainly achieved through incorporation of the additional antigens. Notably, the recombinant
    VlsE of Borrelia garinii strain PBi displayed the highest sensitivity of all antigens tested for IgG detection and is also one of the most useful antigens for IgM.

    Due to its excellent sensitivity and specificity combined with ease of evaluation, this line immunoblot assay offers a useful improvement in serodiagnosis of Lyme borreliosis.

    PMID: 16081885 [PubMed - in process]