NEW research - 'acute' & later stage LYME & short treatment

Discussion in 'Lyme Disease Archives' started by victoria, Mar 9, 2008.

  1. victoria

    victoria New Member

    Linking Human Animal Biomedical Research To Benefit Both

    Persistence of Borrelia burgdorferi
    Following Antibiotic Treatment in Mice

    Antimicrobial Agents and Chemotherapy, published online ahead of print on 3 March 2008

    http://dx.doi.org/10.1128/AAC.01050-07

    Emir Hodzic, Sunlian Feng, Kevin Holden, Kimberly J. Freet, and Stephen W. Barthold

    *Abstract*

    The effectiveness of antibiotic treatment was examined in a mouse model of Lyme borreliosis.

    Mice were treated with ceftriaxone or saline for one month, commencing during the early (3 weeks) or chronic (4 months) stages of infection with Borrelia burgdorferi. Tissues from mice were tested for infection by culture, polymerase chain reaction (PCR), xenodiagnosis, and transplantation of allografts at 1 and 3 months after completion of treatment. In addition, tissues were examined for spirochetes by immunohistochemistry.

    In contrast to saline-treated mice, mice treated with antibiotic were consistently culture-negative, but tissues from some of the mice remained PCR-positive, and spirochetes could be visualized in collagen-rich tissues. Furthermore, when some of the antibiotic treated mice were fed upon by Ixodes scapularis ticks (xenodiagnosis), spirochetes were acquired by the ticks, based upon PCR, and ticks from those cohorts transmitted spirochetes to naïve SCID mice, which became PCR-positive, but culture-negative.

    Results indicated that following antibiotic treatment, mice remained infected with non-dividing but infectious spirochetes, particularly when antibiotic treatment was commenced during the chronic stage of infection.

    http://dx.doi.org/10.1128/AAC.01050-07 Copyright © 2008 by the American Society for Microbiology.

    AND

    A profile of Stephen Barthold, co-author of the above study

    LINKING HUMAN ANIMAL BIOMEDICAL RESEARCH TO BENEFIT BOTH UCDavis Medicine, Spring 2008

    A University of California-Davis School of Medicine publication for alumni, friends and physicians

    ucdmc.ucdavis.edu/ucdavismedicine/issues/Spring2008/features/3.html

    No one knew what Lyme disease was when Stephen Barthold's daughter was diagnosed with it in 1978. At the time, Barthold and his family were living in Connecticut, the state where the cause of the tickborne disease would be identified four years later.

    While a course of antibiotics for an unrelated infection cured his daughter, the father remained intrigued. Today, with 30 years of research and more than 100 papers on Lyme disease under his belt, Barthold, a veterinary pathologist with a joint appointment in the schools of Medicine and Veterinary Medicine, is recognized as one of the leading authorities on how the Lyme bacterium interacts with the hosts it infects.

    ...But, says Barthold, "our work has shown that in the absence of antibiotic treatment, 100% of animals infected with Lyme bacteria remain infected even though they have a perfectly functional immune response."

    Working with mice, Barthold has found that the bacteria, Borrelia burgdorferi, "literally integrate themselves into collagen tissue. They colonize little spots here and there: one joint, but not another; nervous tissue; the heart. It varies from individual to individual, which explains the disease's highly variable clinical manifestations."

    In a study to be published later this year, Barthold outlines his discovery that even after long-term antibiotic treatment, bacteria hidden in collagen tissue are still viable and infectious. "We're trying to be careful in what we claim," he says, "but these findings will be controversial."

    ---------------------

    I wonder what would happen if he'd added in Flagyl to break up cysts AND extended treatment time for later-treated mice. Hope he does that next! But it certainly shows that short-term treatment doesn't work for late-determined Lyme and not very well for even 'acute' cases; AND yet again how diverse symptoms can be!





    [This Message was Edited on 03/09/2008]
  2. foggyfroggy

    foggyfroggy Guest

    Well, that's kinda depressing isn't it? Well at least it's more ammo to fire at those who don't believe in long-term treatment.

    Gretchen
  3. victoria

    victoria New Member

    It's pretty much what I've read about other research showing, plus hearing others' experiences... so I wasn't surprised. That's why I talk about treating lyme etc, as "beating it into submission".

    I am just hoping they'll follow up with longer term, like 18 months at least, abx...

    And also research looking for what happens when lyme is combined with other commonly transmitted infections like bartonella or babesia, etc. in animal models...

    I just saw on (Discovery channel maybe?) a 60 minute show on Gulf War Syndrome. They concentrated mostly on the aspect of exposure to depleted uranium inhaled via smoke - some new type of chromosome tests clearly shows chromosomal damage. They said ultimately the best thing these vets who were tested could do was to build their immune system as best as possible...

    I think besides treatment for lyme etc, that's also true for any one with these stealth pathogens, viral or bacterial.

    all the best,
    Victoria