Parvovirus B19: Host Gene Variability

Discussion in 'Fibromyalgia Main Forum' started by tansy, Nov 16, 2005.

  1. tansy

    tansy New Member

    Kerr, J. R.

    Pathogenesis of Parvovirus B19 Infection: Host Gene Variability, and
    Possible Means and Effects of Virus Persistence.

    Journal of Veterinary Medicine Series B 52 (7-8), 335-339.

    Since conducting follow-up studies of patients with acute symptomatic
    parvovirus B19 infection which showed that a significant proportion of
    patients develop prolonged arthritis and chronic fatigue syndrome (CFS), we
    have become interested in the mechanisms of this phenomenon.

    We showed that these cases have high levels of pro-inflammatory cytokines in their
    circulation and that this correlates with the symptoms. However, the
    underlying mechanisms were not apparent, and we have used various approaches to begin studying this phenomenon.

    DNA polymorphisms were looked for and
    several were shown to be more common in these subjects compared with
    controls; these occur within genes of both the immune response [human
    leucocyte antigen (HLA)-DRB1, HLA-B, transforming growth factor (TGF)-รข1]
    and those involved in several other cellular functions (predominantly the
    cytoskeleton and cell adhesion).

    Interestingly, one particular
    single-nucleotide polymorphism (SNP) which is associated with symptomatic
    B19 infection occurs in the Ku80 gene which has recently been shown to be a
    B19 co-receptor.

    B19 persistence is probably the key to this phenomenon, and
    some new data are presented on short regions of sequence homology (17-26 bp)
    between human, mouse and rat parvoviruses and their respective hosts which
    occur in many host genes. This homology may provide a foothold for virus
    persistence and may also play a role in the genesis of disease through gene
    disruption.

    Finally, we used microarrays and TaqMan real-time polymerase
    chain reaction in 108 normal persons to study human gene expression in
    persons who are B19-seropositive versus B19-seronegative (age- and
    sex-matched) to examine the hypothesis that gene regulation may be altered
    in subjects harbouring the B19 virus DNA.

    Six genes were found to be
    differentially expressed with roles in the cytoskeleton (SKIP, MACF1, SPAG7,
    FLOT1), integrin signalling (FLOT1, RASSF5), HLA class III (c6orf48), and
    tumour suppression (RASSF5). These results have implications not only for
    B19 but also for other persistent viruses as well and confirmation is
    required.

    In conclusion, these disparate findings contribute to our
    understanding of the pathogenesis of B19 disease. We are using these studies
    as a starting point to study the phenomenon of chronic immune activation
    following B19 infection.
    [This Message was Edited on 11/16/2005]
  2. kcollins

    kcollins New Member

    Thanks for posting this Tansy. Can you or anyone else interpret this for me? I became ill with FMS in 1996 after parvo virus B19 infection and have never recovered. When I brought up IVIG treatment I had read about as a treatment for this my rheum. scoffed at the idea since "I wouldn't still be infected with the virus 9 yrs later". After that I was quite defeated and only recently started wondering again what I can do to get better. I got a positive test result for neurotoxins on the VCS test on Dr. Shoemakers site and I guess that has me wondering again if I have tried everything I can to get better.
    Take care,
    Kathy
  3. kcollins

    kcollins New Member

  4. Bailey-smom

    Bailey-smom New Member

    I hope this does not sound too ignorant but this is the first time I have heard that humans can have Parvo and Kathy, you are the first person I have known.

    We raise labs and vaccinate for parvo as it can kill animals so it makes me wonder โ€“ I noticed the article you have above, Tansy, is from the Journal of Veterinary Medicine โ€“ do humans & animals get the same strain?

    We have never had it at our home but we have talked to others who have and it is VERY contagious. At least the kind that animals get โ€“ you have to wash everything with bleach and other animals can not be around for 2 months or more.

    But now that I am typing it must not be the same because humans are allowed in that area during that time. I wonder what the differences are.

    I guess I, like Kathy, would like more info if someone has it.

    Thanks bunches!

    Kelly
  5. atpeace

    atpeace New Member

    Thanks for posting this information! I found a study linking Parvo B-19 to FMS a couple years ago. VERY interesting stuff.

    In humans, Parvo B-19 is usually called "Fifths Disease" (or, slapped cheek disease). It's typically considered a childhood illness, and kids' only symptom is usually the bright red cheeks and maybe a fever. In adults, it can cause a lacey rash all over the body, fever, and body aches. It can last a long time in adults, with symptoms showing up off and on for possibly a year or more.

    I remember reading that many, if not most, people who are infected with Parvo B-19 as kids don't even KNOW it. They don't get symptoms or they don't notice them.

    It's certainly something worth looking into for the specialists, IMO.

    Lori
    [This Message was Edited on 11/17/2005]
  6. kcollins

    kcollins New Member

    My understanding is that Parvo-virus infection comes in different forms that effect different species (human, dog, rodent) and a human doesn't catch it from animals. I caught this from my daughter when it was going around her school. It is mild in most cases but can cause long term illness in some people according to my rheumatologist. When I first was ill he asked me if anyone at home was sick and at first I said no because my daugh. illness was so mild. He then specifically asked about fifth disease and I remembered my daugh. had it. He tested me at that time and found I had very high antibodies showing active infection. I waited for the 1 year and then the 2 yr mark to come hoping my symptoms would subside but 9 years later, I am no better. I wish I understood this latest study more, I'm not sure if it is saying the virus persists or if it triggers an auto-immune reaction.
  7. atpeace

    atpeace New Member

    You know, I was never tested for antibodies after my Fifths Disease experience. I wonder if I still have them in my body, even after 11-12 years.

    I had the Fifths Disease about a year or so before the FMS symptoms started, as nearly as I can remember. The fog is thick lately. ;)

    As I said, it certainly seems like something the specialists should continue to follow up on.

    Lori
    [This Message was Edited on 11/17/2005]
    [This Message was Edited on 11/17/2005]
  8. tansy

    tansy New Member

    I posted this article because a small % of PWME/CFS have tested postive for this parvo virus. Like other infections implicated in these DDs it can trigger an immune response that stays switched on; or the infections becomes chronic which will also lead to immune system activation markers being found. Parvo virus B19 also has the potential to alter gene expressions, which are referred to here.

    I have not researched this particular infection so cannot enlighten you further.

    Dr Jonathan Kerr, the researcher who has already indentified 16 changed gene expressions in PWME/CFS, has been discussing identifying specific viruses so that the best Tx is made available. If funding allows he will hopefully find a better means of identifying individual infections which continue to trigger symptoms and health issues.

    Dr Kerr also demonstrated through his work a similar changed gene expression related to organo phosphates that is present in GWS/I; so infections are not be the only problem in these DDs.

    Tansy[This Message was Edited on 11/19/2005]