Question on the "30's" bands

Discussion in 'Lyme Disease Archives' started by vcamlin, Jun 15, 2008.

  1. vcamlin

    vcamlin New Member

    On the 2 bands that the cdc won't count....something in the 30's I think.

    One is for the herpes virus and one is for Mono right? I've never had mono, ever. I've never had a herpes outbreak. But I guess I could still be a carrier. My regular lab can check for those things right?
  2. bunnyfluff

    bunnyfluff Member

    bands 31 & 34 are SO SPECIFIC to Lyme Bb, that the first human vaccine was made from them! The CDC has left them out, b/c anyone that had the vaccine could potentially test positive on those bands.


    None of these bands has anything to do with herpes or mono.

    Please read this explaination of the bands:




    WESTERN BLOT

    1. Reflects antibody response to specific Bb antigens

    2. Different sensitivities and specificities of the bands

    3. Some bands are potentially seen in different bacteria- “nonspecific bands”

    4. Some bands are specific to spirochetes

    5. Some bands are specific to Bb

    6. Specific: 18, 23-25, 28, 31, 34, 37, 39, 58, 83 & 93

    7. Spirochetes in general: 41 (flagellum)

    8. First immune response if present is usually 41 and 23 KD bands

    9. Response to the 31 KD proteins is not usually seen for a year after initial infection

    CDC IGG WB CRITERIA

    1. IGG WB 5 of the 10 bands (18,23,28,30,39,41,45,58,66)

    2. Criteria based on early Lyme

    3. IGENEX adds 3 specific bands (31,83 and 34) and 3 non-specific bands (22,37,73)

    CDC IGM WB CRITERIA

    1. IGM WB 2 of the 3 bands 23, 39, 41

    2. IGENEX adds 3 specific bands (31,34 and 83) and 3 non-specific bands (22,37,73)

    REVISED CRITERIA WITH WB

    1. IGG WB: 2 specific band criteria has demonstrated improved sensitivity and maintained specificity

    2. Can diagnosis Lyme if any one band (IgG or IgM) of 18, 23, 28, 39 or 58 kDa or if any 2 or more of the following bands are present; 30,45,41 and 93

    3. If negative or require further confirmation, can obtain IGENEX WB (adds specific bands of 31, 34 an 83, which are typically seen in chronic disease)

    4. Positive if any one band of 18, 23, 28,31,34, 39, 58 or 83

    5. If positive for Borrelia on any test, consider testing for neurotoxins

    6. Consider testing for co-infections

    7. Check for coagulation defect (See Hypercoaguable State in CFS and FM)

    Under point #2 it says Lyme can be diagnosed if any two of these bands are present. I have band 30 & 41 ++.

    Under point #4 it says that Lyme can be diagnosed if any of these one bands are present. I have band 58 ++.


    I hope this helps clear up any confusion,

    Bunny
  3. buttercakes

    buttercakes New Member

    Great Post, This clears up alot of questions I had about the westerm blot.take care, Sandie
  4. munch1958

    munch1958 Member

    Chicken pox is a herpes virus which can morph into shingles.

    Epstein-Barr is another herpes viruses. There are lots of them.

    I agree with Bunny's post. 100% good info there!