Scotch study: NEW bands on Western Blot Positives

Discussion in 'Lyme Disease Archives' started by victoria, Jul 5, 2010.

  1. victoria

    victoria New Member

    More specific bands in the IgG western blot in sera from Scottish patients with suspected Lyme borreliosis. Evans R, Mavin S, McDonagh S, Chatterton JM, Milner R, Ho-Yen DO.

    Journal of Clinical Pathology, published online before print, June 30, 2010. http://dx.doi.org/10.1136/jcp.2010.076307

    Abstract

    Aims

    To identify further Western blot bands that may be specific in the diagnosis of Lyme borreliosis.

    Methods

    The Borrelia burgdorferi antibody profiles of 270 western blot positive patients and 241 western blot negative patients from 2008 were examined.

    Results

    27 different non-specific bands were detected in both groups. Six of 27
    (22%) of the non-specific bands were detected significantly more in the western blot positive patients compared to the western blot negative patients (20 kDa, p<0.0001; 28 kDa, p<0.002; 36 kDa, p<0.002; 37 kDa, p<0.007; 48 kDa, p<0.023; 56 kDa, p<0.028; two-tailed F test).

    Conclusion

    Results suggest that the 20, 28 and 48 kDa bands should be regarded as specific.

    http://dx.doi.org/10.1136/jcp.2010.076307


    [This Message was Edited on 07/05/2010]