Tigecycline inneffective for chronic/'persistant' Lyme (study)

Discussion in 'Lyme Disease Archives' started by victoria, Dec 12, 2009.

  1. victoria

    victoria New Member

    Ineffectiveness of Tigecycline Against Persistent Borrelia burgdorferi
    Stephen W. Barthold, Emir Hodzic, Denise M. Imai, Sunlian Feng, Xiaohua
    Yang, and Benjamin J. Luft

    Antimicrobial Agents and Chemotherapy, published online ahead of print
    on 7 December 2009.
    http://dx.doi.org/10.1128/AAC.00788-09

    Abstract

    The effectiveness of a new first in class antibiotic, tigecycline
    (glycylcycline), was evaluated during the early dissemination (1 week),
    early immune (3 weeks), or late persistent (4 months) phases of Borrelia
    burgdorferi infection in C3H mice.

    Mice were treated with high or low doses of tigecycline, saline
    (negative-effect controls), or a previously published regimen of
    ceftriaxone (positive-effect controls). Infection status was assessed at
    3 months after treatment by culture, quantitative ospA real-time PCR,
    and subcutaneous transplantation of joint and heart tissue into SCID
    mice. Tissues from all saline-treated mice were culture- and ospA
    PCR-positive; tissues from all antibiotic-treated mice were
    culture-negative; and some of the tissues from most of the mice treated
    with antibiotics were ospA PCR-positive, although the DNA marker load
    was markedly decreased compared to saline-treated mice.

    Antibiotic treatment during the early stage of infection appeared to be
    more effective than treatment that began during later stages of
    infection. The viability of non-cultivable spirochetes in
    antibiotic-treated mice (demonstrable by PCR) was confirmed by
    transplantation of tissue allografts from treated mice into SCID mice,
    with dissemination of spirochetal DNA to multiple recipient tissues, and
    by xenodiagnosis, including acquisition by ticks, transmission by ticks
    to SCID mice, and survival through molting into nymphs and then into adults.

    Furthermore, PCR-positive heart base tissue from antibiotic-treated mice
    revealed RNA transcription of several B. burgdorferi genes. Results
    extended previous studies with ceftriaxone, indicating that antibiotic
    treatment is unable to clear persisting spirochetes, which remain viable
    and infectious, but are non- or slowly dividing.

    http://dx.doi.org/10.1128/AAC.00788-09
  2. munch1958

    munch1958 Member

    But all of us know that first hand... It's so hard to kill. Was this Abx supposed to be the future for Lyme patients according to Pam W's book "Cure Unknown"? I had high hopes for it.

    Anyone tried Ketek? According to Dr B's last update it's supposed to be effective at killing spirochetes but it hard on the liver.
  3. ChuckNBerkeley

    ChuckNBerkeley New Member

    I found a pdf of an IDSA slide presentation last fall that appears to describe more details of this.

    Tigecycline was administered for 10 days!
  4. victoria

    victoria New Member

    Linda I don't remember... :(

    Chuck, I wonder what 10 days equals in a mouse's life, comparatively to ours; any idea?