XMRV-No Contamination, Still No Replication!

Discussion in 'Fibromyalgia Main Forum' started by Wildaisy, Mar 14, 2011.

  1. Wildaisy

    Wildaisy New Member

    Since the publication of Lombardi et al. in Science magazine over sixteen months ago, the ME community has watched one group after another attempt to refute the finding of an association between ME, and the gammaretrovirus XMRV. Even with the publication of a confirmatory study, Lo et al, in August 2010, the story fed to the media and public, was that polytropic sequences must be considered to be a separate retrovirus to XMRV. Yet, XMRV is a polytropic/xenotropic hybrid, and therefore Lo et al. did indeed confirm the findings of the original paper.

    Others are now attempting to claim that the findings must be the result of contamination. They have proposed that this contamination resulted from a prostate cancer cell line called 22Rv1, which is known to be infected with what looks like XMRV. Yet, Lombardi et al and Lo et al did not use this cell line, the labs involved have never worked with mice, and mouse contamination has been rigorously ruled out in their samples, both by the authors and the CDC.

    These researchers believe that as the cell line has been passaged through mice, XMRV was generated from two mouse viruses, a process called recombination. Using a new assay (test), they examined several generations of the cell line for XMRV, and were unable to detect XMRV in the earlier generations. However, it is unknown whether those assays were capable of detecting XMRV in those tissues if present. Furthermore, the later passages were stimulated with testosterone, which would raise the titre to a level where less clinically sensitive assays could detect XMRV. This research has therefore confirmed that XMRV is a retrovirus found in humans. These viruses are known to cause such pathology in other hosts; there is no reason to suspect that they do not do so in humans.

    The IMEA is therefore clear that research and funding are urgently needed. We are calling on all Governments to prioritise XMRV research and ban people with ME from donating blood. In particular, labs that have proven assays, such as the Whittemore Peterson Institute in the USA and IrsiCaixa in Spain, should now be supported with funding and assistance to quickly develop better and more reliable detection methods, and to investigate the pathogenesis and the potential need for treatment of this retrovirus in not only the diseases currently found to be associated with XMRV, but in those also suspected to have a retroviral cause. Delay is unacceptable considering the potential consequence this retrovirus may have on the human population.

    CEO: Louise Gunn, United Kingdom

    14 March 2011

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